Cell Therapy: Stem Cell Transplantation, Gene Therapy, and Cellular Immunotherapy

Part I. Aspects of Hematopoiesis: 1. Overview of hematopoiesis and review of cells needed for hematopoietic reconstitution; 2. Review of cells important for adoptive cellular immunotherapy; 3. Potential uses of defined cell populations; 4. Progenitor cell assays; 5. Flow cytometric techniques; 6. Principles of gene therapy; 7. Sources of progenitor cells; 8. Chemotherapy approaches to mobilization of progenitor cells; 9. Cytokine approaches to mobilization of progenitor cells; 10. Stem cell isolation; 11. Methods of cell selection using monoclonal antibodies; 12. Magnetic approaches to cell separation; 13. Growth factors and ex-vivo expansion of hematopoietic progenitor cells; 14. Bioreactors for expansion; 15. Purging of malignant cells; Part II. Current Clinical Approaches: 16. What can be achieved without cell therapy; 17. Clinical potential of hematopoietic growth-factor support for high-dose chemotherapy; 18. Review of high-dose chemotherapy requiring cellular support in solid tumors; 19. Review of high-dose chemotherapy requiring cellular support in hematological malignancies; 20. Review of the potential use of cell therapy in leukemia; Part III. Future Clinical Applications: 21. Clinical gene therapy; 22. Correction of inborn errors; 23. Cellular therapy approaches to the treatment of AIDS; Part IV. Experimental and Clinical Cellular Immunotherapy: 24. Adoptive immunotherapy with T cells; 25. Cellular adoptive immunotherapy with natural killer cells; 26. Autologous tumor cell vaccines; 27. Allogeneic cellular vaccines against tumors; 28. Neutrophil transfusions for treatment of infections; 29. Experimental and clinical cellular immunotherapy in bone-marrow transplantation; 30. Donor lymphocyte therapy in bone-marrow transplantation; 31. Graft-versus-leukemia effect in bone-marrow transplantation.