Sample Preparation Techniques for Soil, Plant, and Animal Samples

The Sample Preparation Techniques for Environmental, Plant, and Animal Samples handbook is a collection of best practices, recipes and theoretical information aimed at anyone who works with any type of molecular biology, proteomics, or metabolomics research involving diffi cult and tough-to-process samples, and thus is exposed to the seemingly unbreakable bottleneck of sample preparation. Th is book is most useful to researchers preparing nucleic acids and proteins from environmental (e. g. , soil, marine, and wastewater, feces) and tough microbiological (e. g. , spores, yeasts, gram positive bacteria) samples, as well as solid tissue samples from plants and animals. This book is the first comprehensive piece of literature dealing with applications of bead beating technology and other types of mechanical homogenization sample preparation. This protocol volume will be the first publication to comprehensively cover entire spectrum of molecular biology sample preparation techniques commonly used to extract and isolate nucleic acids, proteins and other molecules of interests, from plants, animals and environmental samples and similar difficult samples. Sample preparation is considered to be the first and most significant bottleneck in the molecular biology lab workflow, and it is very peculiar fact that while there is an estimated $3. 6B market (Bcc Research Report ( BIO089A, 2010) in sample preparation consumables and equipment for Genomics, Proteomics, and Epigenetics; and that approximately 180, 000 people worldwide are performing sample preparation techniques in the lab daily, there is no single book dealing exclusively with this topic. The proposed book will present an in depth literature review of current state-of-the-art knowledge in all aspects of molecular biology related sample preparations methods, techniques and applications, and provide a comprehensive collection of practical recipes and advice on the most efficient methods of processing the most common sample types in agriculture, plant science, soil science, environmental science, and animals research. The book will be an essential tool for demystifying time consuming, and often the last remaining bottleneck process in molecular biology sample preparation and will be of special interest to all lab technicians bench scientists, graduate students, postdocs, as well as other senior scientists working with DNA, RNA and Proteins in such a diverse areas ranging from plant, soil, environmental science, animal research, as well as to more applied fields of quality control and law enforcement.