This volume not only discusses various common biobanking topics, it also delves into less-discussed subjects such as what is needed to start a biobank, training of new biobanking personnel, and ethnic representation in biospecimen research. Other chapters in this book span practical topics including: disaster prevention and recovery; information technology; flora and fauna preservation including zoological fluid specimen photography; surgical and autopsy biobanking; biobanking of bodily fluids; biosafety; cutting frozen sections; immunohistochemistry; nucleic acid extraction; and biospecimen shipping. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Unique and comprehensive, Biobanking: Methods andProtocols is a valuable resource for novice and practicing biobankers, and for end-user researchers. This book aims to bring new insight into the field and expand on current biomedical biobanking studies.
Inhaltsverzeichnis
Sustainability in Biobanking- An Introduction to Starting a Biobank. - An Introduction to Hardware, Software, and Other Information Technology Needs of Biomedical Biobanks. - Disaster Prevention and Recovery. - Minority Participation in Biobanks: An Essential Key to Progress. - Orientation and Training of New Biobank Personnel. - Procurement and Storage of Surgical Biospecimens. - Autopsy Biobanking: Biospecimen Procurement, Integrity, Storage, and Utilization. - Procurement, Storage, and Use of Blood in Biobanks. - Procurement, Transportation, and Storage of Saliva, Buccal Swab, and Oral Wash Specimens. - Biobanking of Cerebrospinal Fluid. - Biobanking of Urine Samples. - Procurement and Storage of Pleural and Peritoneal Fluids for Biobanking. - The Fluid Preservation of Zoological Specimens. - Photographing Fluid Preserved Specimens. - Casts of Fluid Preserved Specimens. - Collection and Preservation of Terrestrial Arthropods. - Procurement and Preservation of Plants. - Biosafety and Biohazards: Understanding Biosafety Levels and Meeting Safety Requirements of a Biobank. - Decontamination of Biobank Facilities. - Laboratory Safety: Chemical and Physical Hazards. - Making Formalin-Fixed Paraffin Embedded Blocks. - Microtomy: Cutting Formalin-Fixed Paraffin Embedded Sections. - Performing and Cutting Frozen Sections. - An Introduction to the Performance of Immunohistochemistry. - An Introduction to Performing Immunofluorescence Staining. - Making a Tissue Microarray. - Nucleic Acid Isolation and Quality Control. - Fundamentals of RNA Analysis on Biobanked Specimens. - Nucleic Acid Extraction from Human Biological Samples. - An Overview of DNA Analytical Methods. - Shotgun Proteomic Profiling of Blood Borne Nanoscale Extracellular Vesicles (NsEVs). - Sample Preparation for Transmission Electron Microscopy. - Processing of Primary Patient Tumors and Subsequent Generation of Primary Cell Lines. - Domestic and International Shipping of Biospecimens.
