Selective Autophagy

This volume presents the latest techniques used by researchers to study selective autophagy in a broad range of organisms such as yeast, plants, worms, fruit flies, and mammals. The chapters in this book cover several types of selective autophagy such as mitophagy, aggrephagy, ER-phagy, ferritinophagy, pexophagy, and nucleophagy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and comprehensive, Selective Autophagy: Methods and Protocols is a valuable resource for novice and expert researchers who want to learn more about this interesting and developing field.



Chapter 3 is available open access under a Creative Commons Attribution 4. 0 International License via link. springer. com.

Using Fluorescence Microscopy and Immunoblotting for Monitoring Mitophagy in Budding Yeast. - Methods to Monitor Nucleophagy in Yeast. - Synthetic Dimerization Approaches for In Vivo Studies in
Saccharomyces
cerevisiae. -
Assays to Study Mitophagy in Plants. - Assessing Neuronal Mitophagy during Development in the Nematode
Caenorhabditis elegans. -
In Vivo Monitoring of Nucleophagy in
Caenorhabditis elegans. -
Analysis of Mitophagy Reporters in
Drosophila. -
Studying Selective Autophagy of Protein Aggregates using Particles Induced by Multimerization (PIMs). - Methods for Monitoring ER-phagy. - Quantitative Super-Resolution Imaging of ER-phagy Initiation in Cells. - Fluorescence Methods to Measure Pexophagy. - Methods for Monitoring Mitophagy using mt-Keima. - Monitoring Mitophagy Dynamics in Live Cells. - Methods for Monitoring NCOA4-Mediated Ferritinophagy. - Method to Purify Phase-Separated p62 Bodies using Fluorescence-Activated Particle Sorting. - In Silico Assessment of LIR Motif-Containing Proteins Binding to ATG8-Family Proteins. - High Throughput Screening for LC3/GABARAP Binders Utilizing the Fluorescence Polarization Assay. - Thermodynamic Characterization of LC3/GABARAP:Ligand Interactions by Isothermal Titration Calorimetry. - Determination of hATG8 Binding Selectivity of Autophagy-Interacting Motif (AIM) Peptides Using Native Electrospray Ionization Mass Spectrometry.