Strain Engineering

This book presents powerful new genetic engineering-based strain engineering methods for rational modification of a variety of model organisms. It contains key notes and implementation advice from the experts.

Bacterial Genome Reengineering. - Targeted Chromosomal Gene Knockout using PCR Fragments. - Scarless Chromosomal Gene Knockout Methods. - Random Chromosomal Gene Disruption In vivo using Transposomes. - Genome Engineering using Targeted Oligonucleotide Libraries and Functional Selection. - Microarray-based Genetic Footprinting Strategy to Identify Strain Improvement Genes after Competitive Selection of Transposon Libraries. - Optimization of Synthetic Operons using Libraries of Post-Transciptional Regulatory Elements. - Marker Free Chromosomal Expression of Foreign and Native Genes in E. coli. - Array-based Synthetic Genetic Screens to Map Bacterial Pathways and Functional Networks in Escherichia coli. - Assembling New E. coli Strains by Transduction using Phage P1. - Yeast Bioinformatics and Strain Engineering Resources. - Delete and Repeat: A Comprehensive Toolkit for Sequential Gene Knock-out in the Budding Yeast Saccharomyces cerevisiae. - Genome-wide Transposon Mutagenesis in Saccharomyces cerevisiae and Candida albicans. - Signature-tagged Mutagenesis to Characterize Genes Through Competitive Selection of Barcoded Genome Libraries. - Global Strain Engineering by Mutant Transcription Factors. - Genomic Promoter Replacement Cassettes to Alter Gene Expression in the Yeast Saccharomyces cerevisiae. - Microbial Genome Analysis and Comparisons: Web-based Protocols and Resources. - Plasmid Artificial Modification: A Novel Method for Efficient DNA Transfer into Bacteria. - Broad-host-range Plasmid Vectors for Gene Expression in Bacteria. - Simple Method for Introducing Marker-free Deletions in the Bacillus subtilis Genome. - Transposon-mediated Random Mutagenesis of Bacillus subtilis. - Integrative Food Grade Expression System for Lactic Acid Bacteria. - ClosTron-mediated Engineering of Clostridium. - High Throughput Transposon Mutagenesis of Corynebacterium glutamicum. - Mini-Mu Transposon Mutagenesis of Ethanologenic Zymomonas mobilis. - Engineering Thermoacidophilic Archaea using Linear DNA Recombination. - Targeted Gene Disruption in Koji Mold Aspergillus oryzae. - Selectable and Inheritable Gene Silencing through RNA Interference in the Unicellular Alga Chlamydomonas reinhardtii.