Gene Expression Profiling

Understanding gene expression and how it changes under normal and pathological conditions is essential to our understanding of the fundamentals of cell biology through to the targeted treatment of disease. In Gene Expression Profiling: Methods and Protocols, Second Edition, experts in their particular fields compile detailed protocols for a broad range of techniques, currently available and being further developed, for the analysis of gene expression at the DNA, RNA, and protein levels. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Real-Time PCR and Multiplex Approaches. - Reverse-Transcriptase Polymerase Chain Reaction to Detect Extracellular mRNAs. - Microarray Analysis of mRNAs: Experimental Design and Data Analysis Fundamentals. - Software and Tools for Microarray Data Analysis. - Analysis of Gene Expression as Relevant to Cancer Cells and Circulating Tumor Cells. - Gene Expression Profiling in Formalin-Fixed, Paraffin-Embedded Tissues Using the Whole-Genome DASL Assay. - MicroRNA Expression Analysis: Techniques Suitable for Studies of Intercellular and Extracellular MicroRNAs. - Western Blotting Analysis as a Tool to Study Receptor Tyrosine Kinases. - 2D Gel Electrophoresis and Mass Spectrometry Identification and Analysis of Proteins. - Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis. - Immunohistochemical and Immunofluorescence Procedures for Protein Analysis. - Advanced Microscopy: Laser Scanning Confocal Microscopy. - Isolation of Exosomes for Subsequent mRNA, MicroRNA, and Protein Profiling. - Atomic Force Microscopy (AFM) and High-Content Analysis (HCA): Two Innovative Technologies for Dissecting the Relationship Between Epithelial-Mesenchymal Transition (EMT)-Related Morphological and Structural Alterations and Cell Mechanical Properties.