Biolistic DNA Delivery

Biolistic transfection represents a direct physical gene transfer approach in which nucleic acids are precipitated on biologically inert high-density microparticles (usually gold or tungsten) and delivered directly through cell walls and/or membranes into the nucleus of target cells by high-velocity acceleration using a ballistic device such as the gene gun. Biolistic DNA Delivery: Methods and Protocols provides a comprehensive collection of detailed protocols intended to provide the definitive practical guide for the novice as well as for the advanced gene transfer expert on how to introduce nucleic acids into eukaryotic cells using the biolistic technique. Split into six convenient sections, this detailed volume covers biolistic gene transfer into plants, nematodes, and mammalian cells, both in vitro and in vivo, as well as the use of gene gun-mediated DNA vaccination in various experimental animal models of human diseases, and the description of biolistic delivery of molecules other than nucleic acids. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comparison Between Agrobacterium-Mediated and Direct Gene Transfer Using the Gene Gun. - Transient Gene Expression in Epidermal Cells of Plant Leaves by Biolistic DNA Delivery. - Transformation of Nuclear DNA in Meristematic and Embryogenic Tissues. - Biolistic DNA Delivery to Leaf Tissue of Plants with the Non-Vacuum Gene Gun (HandyGun). - HandGun-Mediated Inoculation of Plants with Viral Pathogens for Mechanistic Studies. - Biolistics-Based Gene Silencing in Plants Using a Modified Particle Inflow Gun. - Biolistic Transformation of Caenorhabditis elegans. - Improved Vectors for Selection of Transgenic Caenorhabditis elegans. - Biolistic Transformation of Brugia malayi. - Biolistic Transfection of Human Embryonic Kidney (HEK) 293 Cells. - Biolistic Transfection of Tumor Tissue Samples. - Biolistic Transfection of Freshly Isolated Adult Ventricular Myocytes. - Biolistic Transfection of Neurons in Organotypic Brain Slices. - Biolistic DNA Delivery to Mice with the Low Pressure Gene Gun. - Chemokine Overexpression in the Skin by Biolistic DNA Delivery. - Enhancement of Gene Gun-Induced Vaccine-Specific Cytotoxic T Cell Response by Administration of Chemotherapeutic Drugs. - Dendritic Cell-Specific Biolistic Transfection Using the Fascin Gene Promoter. - Particle-Mediated Administration of Plasmid DNA on Corneas of BALB/c Mice. - Optimizing Particle-Mediated Epidermal Delivery of an Influenza DNA Vaccine in Ferrets. - Methods for Monitoring Gene Gun-Induced HBV- and HCV-Specific Immune Responses in Mouse Models. - Gene Gun Immunization to Combat Malaria. - Identification of T-cell Epitopes of Mycobacterium tuberculosis with Biolistic DNA Vaccination. - Biolistic DNA Vaccination Against Trypanosoma Infection. - Biolistic DNA Vaccination Against Melanoma. - Biolistic DNA Vaccination Against Cervical Cancer. - Efficiency of Biolistic DNA Vaccination in Experimental Type I Allergy. - Safety Assessment of Biolistic DNA Vaccination. - DiOlistics: Delivery of Fluorescent Dyes into Cells. - Protein Antigen Delivery by Gene Gun-Mediated Epidermal Antigen Incorporation (EAI).