Thin-Layer Chromatography with Flame Ionization Detection

1 General and Methodical.
- 1.1 Principles of Thin-Layer Chromatography and Basic Quantities in the Chromatographic Process.
- 1.1.1 Distribution of the Solute Between the Stationary and Mobile Phases; the Retardation Factor.
- 1.1.2 Flow of the Liquid Phase Through the Layer.
- 1.1.3 The Effect of Diffusion on the Zone Resolution.
- 1.1.4 The Theoretical Plate; Resolution; Separation Number.
- 1.2 Methods of Thin-Layer Chromatography with Flame Ionization Detection (TLC-FID).
- 1.2.1 A Short Description of the TLC-FID Procedure.
- 1.2.2 The TLC-FID Technique.
- 1.2.2.1 The System for Detection in the FID.
- 1.2.2.2 Recorders and Electronic Integrators.
- 1.2.2.3 Accessories for Application, Elution and Drying of Thin Layers.
- 1.2.2.4 Chromarods.
- 1.2.3 Operational Parameters and Procedures.
- 1.2.3.1 Preparation of Chromarods for Analysis.
- 1.2.3.2 Application of the Analysed Mixture to the Rods.
- 1.2.3.3 Elution Solvents.
- 1.2.3.4 Elution.
- 1.2.3.5 Drying of the Eluted Rods Prior to Detection.
- 1.2.3.6 Flame Ionization Detection.- Hydrogen and Air Flow-Rates.- Detector Geometry and Scanning Rate.- The Effect of the Composition of the Analysed Sample.
- 1.2.3.7 Methods for Quantitative Evaluation of the Results of TLC-FID Analyses.- The Internal Normalization Method.- The Absolute Calibration Method.- The Internal Standard Method.
- 1.2.3.8 Reproducibility in TLC-FID.- 2 Specialized Applications.
- 2.1 Applications in Biology, Medicine and Pharmacy.
- 2.1.1 Lipids and Related Substances.
- 2.1.1.1 Isolation of Lipids from Biological Materials.
- 2.1.1.2 Chromatography on Chromarods.
- 2.1.1.3 FID Reproducibility and Response.
- 2.1.1.4 Applications.
- 2.1.2 Steroid Compounds.
- 2.1.3 Vitamins.
- 2.1.4 Alkaloids and Purine Bases.
- 2.1.5 Amino Acids.
- 2.1.6 Terpenes and Resins.
- 2.1.7 Psychotropic Substances.
- 2.1.8 Antipyretics, Analgesics and Hypnotics.
- 2.1.9 Antibiotics.
- 2.1.10Sulphonamides and Sulphanilic Acid.
- 2.1.11Polyamines.
- 2.2 Applications in the Food Industry and Related Fields.
- 2.2.1 Fats, Oils and Related Substances.
- 2.2.2 Phospholipids.
- 2.2.3 Saccharides.
- 2.2.4 Food Additives.
- 2.2.5 Cosmetics.
- 2.3 Applications in the Chemical Industry and Related Fields.
- 2.3.1 Surfactants and Detergents.
- 2.3.2 The Petroleum Industry and Related Fields.
- 2.3.3 The Industrial Production of Technical Emulsions for the Metal, Textile and Leather Industries.
- 2.3.4 The Polymer Industry.
- 2.3.5 Pesticides and Growth Regulators.- Par 3 New Perspectives in TLC on Chromarods.
- 3.1 Increasing the Reproducibility and Sensitivity of the Determination.
- 3.2 New Detection Systems.
- 3.3 New Model of the Iatroscan.- References.

` The present book should inspire chemists, chemical technologists, and instrumentation engineers, especially those in the inter-disciplinary groups, in bringing about further improvements in the technique of TLC-FID. The book is very well prodeced and aptly illustrated. It will be extremely useful to research scientists and technologists who are concerned with the analytical technology of biological, medicinal, pharmaceutical, food and chemical industry products. '
Indian Journal of Chemistry Vol.27A, April 1988