This manual introduces the reader to bacic methods used in the isolation, cloning and analysis of genetic material. The protocols include RT-PCR amplification, gene cloning, hybridization analysis and sequencing of nucleic acids, PCR-based site-specific mutagenesis, analysis of protein DNA-specific interaction, cell-free protein synthesis and product electrophoretic and immunological analysis.
Inhaltsverzeichnis
1 cDNA Synthesis and Cloning. - 2 DNA Sequencing. - 3 RNA In Vitro Synthesis by Phage T7 DNA-Dependent RNA Polymerase. - 4 PCR-Based Site-Specific Mutagenesis. - 5 Analysis of Specific Protein-DNA Interactions. - 6 Gel Electrophoresis, Transfer and Hybridization of Nucleic Acids. - 7 Immunological Methods for Analysis of Recombinant Proteins. - 8 Protein Synthesis in Cell-Free Systems.
