PCR and cDNA Cloning Techniques.- 1 Strategies for cDNA Cling and Mapping RNA Transcripts.- 2 Cloning PCR Products.- 3 Site Directed Deletion, Insertion, and Substitution Using PCR.- 4 Long RT-PCR Cloning - Amplification of Full-Length Enterovirus Genomes.- 5 Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase.- Subtractive and Differential Display Techniques.- 6 Subtractive Hybridization and cDNA Cloning.- 7 Differential Display to Identify Steroid-Induced Genes in Endocrine Cells.- Specialized Cloning and Library Screening Strategies.- 8 Two Hyrid cDNA Cloning.- 9 Yeast One and Two Hyrid cDNA Cloning.- 10 High-Throughput Library Screening by Fluorescent Hybridisations on Gridded Membranes.- 11 Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries.
Inhaltsverzeichnis
PCR and cDNA Cloning Techniques. - 1 Strategies for cDNA Cling and Mapping RNA Transcripts. - 2 Cloning PCR Products. - 3 Site Directed Deletion, Insertion, and Substitution Using PCR. - 4 Long RT-PCR Cloning Amplification of Full-Length Enterovirus Genomes. - 5 Construction of cDNA Libraries from Small Quantities of Total RNA Using Template Switching Catalyzed by M-MLV Reverse Transcriptase. - Subtractive and Differential Display Techniques. - 6 Subtractive Hybridization and cDNA Cloning. - 7 Differential Display to Identify Steroid-Induced Genes in Endocrine Cells. - Specialized Cloning and Library Screening Strategies. - 8 Two Hyrid cDNA Cloning. - 9 Yeast One and Two Hyrid cDNA Cloning. - 10 High-Throughput Library Screening by Fluorescent Hybridisations on Gridded Membranes. - 11 Identification of Cell Targeting Ligands Using Random Peptide-Presenting Phage Libraries.
