NEW EDITION COMING OCTOBER 2016
Benson's Microbiological Applications has been the gold standard of microbiology laboratory manuals for over 30 years. The 77 self-contained, clearly-illustrated exercises, and four-color format with a wealth of added photographs makes this the ideal lab manual. Appropriate for either a majors or non-majors lab course, this manual assumes no prior organic chemistry course has been taken.
Inhaltsverzeichnis
< h2> Part 1 Microscopy< h3> 1 Brightfield Microscopy< h3> 2 Darkfield Microscopy< h3> 3 Phase-Contrast Microscopy< h3> 4 Fluorescence Microscopy< h3> 5 Microscopic Measurements< h2> Part 2 Survey of Microorganisms< h3> 6 Microbiology of Pond Water - Protists, Algae, and Cyanobacteria< h3> 7 Ubiquity of Bacteria< h3> 8 The Fungi: Molds and Yeasts< h2> Part 3 Manipulation of Microorganisms< h3> 9 Aseptic Technique< h3> 10 Pure Culture Techniques< h2> Part 4 Staining and Observations of Microorganisms< h3> 11 Smear Preparation< h3> 12 Simple Staining< h3> 13 Negative Staining< h3> 14 Capsular Staining< h3> 15 Gram Staining< h3> 16 Spore Staining: Two Methods< h3> 17 Acid-Fast Staining: Kinyoun Method< h3> 18 Motility Determination< h2> Part 5 Culture Methods< h3> 19 Culture Media Preparation< h3> 20 Preparation of Stock Cultures< h3> 21 Enumeration of Bacteria: The Standard Plate Count< h3> 22 Slide Culture: Fungi< h2> Part 6 Bacterial Viruses< h3> 23 Determination of a Bacteriophage Titer< h3> 24 A One-Step Bacteriophage Growth Curve< h3> 25 Isolation of Phage from Flies< h3> 26 Phage Typing < h2> Part 7 Environmental Influences and Control of Microbial Growth< h3> 27 Effects of Oxygen on Growth< h3> 28 Temperature: Effects on Growth< h3> 29 pH and Microbial Growth< h3> 30 Water Activity and Osmotic Pressure< h3> 31 Ultraviolet Light: Lethal Effects< h3> 32 The Effects of Lysozyme on Bacterial Cells< h3> 33 Evaluation of Alcohol: Its Effectiveness as an Antiseptic< h3> 34 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method< h3> 35 Evaluation of Antiseptics: The Filter Paper Disk Method< h3> 36 Effectiveness of Hand Scrubbing< h2> Part 8 Identification of Unknown Bacteria< h3> 37 Morphological Study of An Unknown Bacterium< h3> 38 Cultural Characteristics< h3> 39 Physiological Characteristics: Oxidation and Fermentation Stet Reactions< h3> 40 Physiological Characteristics: Hydrolytic and Degradative Stet Reactions< h3> 41 Physiological Characteristics: Multiple Test Media< h3> 42 Use of Bergey's Manual< h2> Part 9 Miniaturized Multitest Systems< h3> 43 Enterobacteriaceae Identification: The API 20E System< h3> 44 Enterobacteriaceae Identification: The Enterotube II System< h3> 45 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System< h3> 46 Staphylococcus Identification: The API Staph System< h2> Part 10 Diversity and Environmental Microbiology< h3> 47 Isolation of an Antibiotic Producer: The Streptomyces< h3> 48 Nitrogen Cycle: Ammonification< h3> 49 Symbiotic Nitrogen Fixation: Rhizobium< h3> 50 Free-Living Nitrogen Fixation: Azotobacter< h3> 51 Denitrification: Paracoccus denitrificans< h3> 52 The Winogradsky Column< h3> 53 Purple Nonsulfur Photosynthetic Bacteria< h3> 54 Sulfate Reducing Bacteria: Desulfovibrio< h3> 55 Bacterial Commensalism< h3> 56 Bacterial Synergism< h3> 57 Microbial Antagonism < h2> Part 11 Applied Microbiology< h3> 58 Bacterial Food Counts< h3> 59 Bacteriological Examination of Water: Most Probable Number Determination< h3> 60 Bacteriological Examination of Water: The Membrane Filter Method< h3> 61 Reductase Test< h3> 62 Temperature: Lethal Effects< h3> 63 Microbial Spoilage of Canned Food< h3> 64 Microbiology of Alcohol Fermentation< h2> Part 12 Bacterial Genetics and Biotechnology< h3> 65 Mutant Isolation by Replica Plating< h3> 66 Bacterial Transformation< h3> 67 Polymerase Chain Reaction for Amplifying DNA< h3> 68 Plasmid Isolation< h2> Part 13 Medical Microbiology< h3> 69 The Staphylococci: Isolation and Identification< h3> 70 The Streptococci and Enterococci: Isolation and Identification< h3> 71 Gram-Negative Intestinal Pathogens< h3> 72 A Synthetic Epidemic< h2> Part 14 Immunology and Serology< h3> 73 Slide Agglutination Test: Serological Typing< h3> 74 Slide Agglutination Test for S. aureus< h3> 75 Slide Agglutination Test for Streptococcus< h3> 76 The Heterophile Antibody Test< h3> 77 Blood Grouping< h4> Appendices