Receptor and Ion Channel Detection in the Brain

Preface. - Production of high-quality antibodies for the study of receptors and ion channels in brain. - Co-immunoprecipitation from brain. - Sub-synaptic membrane fractionation. - Investigation of neurotransmitter receptors in brain slices using cell surface biotinylation. - Single nanoparticle tracking of surface ion channels and receptors in brain cells. - Radioligand binding detection of receptors in brain membranes. - Recombinant Alphavirus-Mediated Expression of Ion Channels and Receptors in the Brain. - Fluorescent ligands and TR-FRET to study receptor-receptor interactions in the brain. - In situ Proximity Ligation Assay to study and understand the distribution and balance of GPCR homo- and heteroreceptor complexes in the brain. - Fluorescent in situ hybridization for sensitive and specific labeling. - Autoradiographic visualization of G protein-coupled receptors in brain. - Analysis of the expression profile and regional distribution of neurotransmitter receptors and ion channels in the central nervous system using histoblots. - Immunohistochemistry for ion channels and their interacting molecules: Tips for improving antibody accessibility. - Localization of GFP-tagged proteins at the electron microscope. - Pre-embedding methods in the localization of receptors and ion channels. - Post-embedding immunohistochemistry in the localization of receptors and ion channels. - High-Resolution localization of membrane proteins by SDS-digested freeze-fracture replica labelling (SDS-FRL). - Application of virus vectors for anterograde tract-tracing and single-neuron labeling studies. - Analysis of synaptic connections using viral vectors at the electron microscopic level. - Morphological and neurochemical characterization of electrophysiologically identified cells. - Using electrophysiology to study synaptic and extrasynaptic ionotropic receptors in hippocampal neurons. - Biophysical methods to analyze direct G-protein regulation of neuronal voltage-gated calcium channels. -Electrophysiological recordings in behaving animals. - Voltammetry in behaving animals. - In vivo brain microdialysis of monoamines. - Calcium transients in single dendrites and spines of pyramidal neurons in vitro. - Dynamic recording of membrane potential from hippocampal neurons by using a FRET-based voltage biosensor. - Determination of GPCR-mediated cAMP accumulation in rat striatal synaptosomes. - GPCR-mediated MAPK/ERK cascade activation in brain slices. - Index.