Auditory and Vestibular Research

Thissecond edition expands upon the previous volume with new and updated chapters. Auditory and Vestibular Research: Methodsand Protocols, Second Edition guides readers through protocols on cellculture, tissue engineering, nanotechnology, high-throughput screening, andphysiology. Chapters on physiology covertechniques that include optical coherence tomography, patch clamping, andphotostimulation of caged neurotransmitters. Written in the highly successful Methods in Molecular Biology seriesformat, chapters include introductions to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritativeand cutting-edge, Auditoryand Vestibular Research: Methods and Protocols, Second Edition aims to ensure successfulresults in the further study of this vital field.

Helios®
Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent
Updates. - Tol2-Mediated
Delivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation. - A Rapid,
Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation Enables
Efficient Gene Transfer to the Developing Mouse Inner Ear. - Generation
of Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannoma
and Meningioma. - RNA
Extraction from Xenopus Auditory and Vestibular Organs for Molecular Cloning
and Expression Profiling with RNA-Seq and Microarrays . - Yeast
Two-Hybrid Screening to Test for Protein-Protein Interactions in the Auditory
System. - Multiplexed
Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to
Auditory Research. - Protein
Quantification of the Developing Cochlea by Mass Spectrometry. - Ultrastructural
Identification and Colocalization of Interacting Proteins in the Murine Cochlea
by Post-Embedding Immunogold Transmission Electron Microscopy. - Surface Plasmon Resonance (SPR) Analysis of
Binding Interactions of Inner-Ear Proteins. - The Single-Molecule Approach to Membrane
Protein Stoichiometry. - Visualization of Live Cochlear Stereocilia at
a Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy. - Design and Construction of a Cost-Effective
Spinning Disk System for Live Imaging of Inner Ear Tissue. - Neuroanatomical Tracing Techniques: History,
State of the Art, and Future Developments. - Detection of Excitation and Inhibition in the
Auditory System Using Fluorescence Immunohistochemistry and High Resolution
Fluorescence Microscopy. - Rapid and Semi-Automated Extraction of
Neuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks. -Organotypic Culture of the Mouse Cochlea from
Embryonic Day 12 to the Neonate. - Quantifying Spiral Ganglion Neurite and
Schwann Behavior on Micropatterned Polymer Substrates. - The Use of Human Wharton s Jelly Cells for
Cochlear Tissue Engineering. - Nanotechnology in Auditory Research: Membrane
Electromechanics in Hearing. -
Overview of Nanoparticle Based Delivery for Treatment of Inner Ear Disorders. - Development of Cell-based High-Throughput Chemical
Screens for Protection Against Cisplatin-induced Ototoxicity. -Profiling Specific Inner Ear Cell Types Using
Cell Sorting Techniques. - Optical Coherence Tomography to Measure
Sound-Induced Motions Within the Mouse Organ of Corti In Vivo. - Method for Dissecting the Auditory Epithelium
(Basilar Papilla) in Developing Chick Embryos. - Whole Cell Patch Clamp Recording of Mouse and
Rat Inner Hair Cells in the Intact Organ of Corti. - Glass Probe Stimulation of Hair Cell
Stereocilia. - A Walkthrough of Nonlinear Capacitance
Measurement of Outer Hair Cells. - In Vitro Functional Assessment of Adult
Spiral Ganglion Neurons (SGNs). - Mapping Auditory Circuits with
Photostimulation of Caged Glutamate.