RNA Detection

The Secret Life of RNA: Lessons from Emerging Methodologies. - Quantification of 2' -O-Me Residues in RNA using Next-Generation Sequencing (Illumina RiboMethSeq Protocol). - Identifying the m
⁶
A Methylome by Affinity Purification and Sequencing. - PARIS: Psoralen Analysis of RNA Interactions and Structures with High Throughput and Resolution. - Axon-TRAP-RiboTag: Affinity-Purification of Translated mRNAs from Neuronal Axons in Mouse
In Vivo. -
LCM-Seq: A Method for Spatial Transcriptomic Profiling using Laser Capture Microdissection Coupled with PolyA-Based RNA Sequencing. - Spatial Transcriptomics: Constructing a Single-Cell Resolution Transcriptome-Wide Expression Atlas. - Single mRNA Molecule Detection in
Drosophila. -
Detection and Automated Analysis of Single Transcripts at Subcellular Resolution in Zebrafish Embryos. - Super-Resolution Single Molecule FISH at the
Drosophila
Neuromuscular Junction. - Detection of mRNA and Associated Molecules by ISH-IEM on Frozen Sections. - Hybridization Chain Reaction for Direct mRNA Detection without Nucleic Acid Purification. -
In Situ
Detection of MicroRNA Expression with RNAscope Probes. - Padlock Probes to Detect Single Nucleotide Polymorphisms. - Quantifying Gene Expression in Living Cells with Ratiometric Bimolecular Beacons. - Optimizing Molecular Beacons for Intracellular Analysis of RNA. - Live Imaging of Nuclear RNPs in Mammalian Complex Tissue with ECHO-liveFISH. -
In Vivo
Visualization and Function Probing of Transport mRNPs using Injected FIT Probes. - Visualizing RNA in Live Bacterial Cells using Fluorophore- and Quencher-Binding Aptamers. - Method for Imaging Live-Cell RNA using an RNA Aptamer and a Fluorescent Probe. - RNA Live Imaging in the Model Microorganism
Ustilago maydis. -
Real-Time Fluorescence Imaging of Single-Molecule Endogenous Non-Coding RNA in Living Cells. - Live Imaging of mRNA Synthesis in
Drosophila. -
Imaging Newly Transcribed RNA in Cells by Using a Clickable Azide-Modified UTP Analog. - Detection of the First Round of Translation: The TRICK Assay. - Imaging Translation Dynamics of Single mRNA Molecules in Live Cells. - Systematic Detection of Poly(A)
⁺
RNA-Interacting Proteins and Their Differential Binding. - Isolation and Characterization of Endogenous RNPs from Brain Tissues. - Individual Nucleotide Resolution UV Cross-Linking and Immunoprecipitation (iCLIP) to Determine Protein-RNA Interactions. - RNA Tagging: Preparation of High-Throughput Sequencing Libraries. - RAP-MS: A Method to Identify Proteins that Interact Directly with a Specific RNA Molecule in Cells.