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Produktbild: Flow Cytometry | Michael G. Ormerod
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Produktbild: Flow Cytometry | Michael G. Ormerod

Flow Cytometry

A Practical Approach

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Flow cytometry is a technique widely used in biological research and in diagnostic medicine. Flow cytometers are found in most biological research institutions and most clinical laboratories in larger hospitals.
Flow Cytometry 3/e is intended as a handbook for every laboratory that has a bench-top flow cytometer or a fluorescence activated cell sorter. It is an introduction and guide to those new to the field and a first point of reference for experienced practitioners who want to investigate a new
technique. The chapter of immunophenotyping - the most important clinical application of flow cytometry - has been strengthened by the addition of a chapter on quality control in the clinical laboratory. The utility of the book in a clinical laboratory has been further enhanced by the addition of
a chapter covering ten other clinical applications. Flow cytometry has found increasing application in the field of apoptosis research. A new chapter has been added to cover this important topic. Every flow cytometry laboratory can't afford not to have a copy on the shelf as a first point of
reference. The book is not fully comprehensive but it does aim to cover over 90% of the applications of flow cytometry in mammalian biology.

Inhaltsverzeichnis

  • Introduction to the principles of flow cytometry

  • Fluorescence and fluorescence technology

  • Preparing of suspensions of single cells

  • Flow sorting

  • Immunofluorescence of surface markers

  • Analysis of DNA - general methods

  • Further clinical applications

  • Quality control in the clinical laboratory

  • Measurement of cytoplasmic and nuclear antigens

  • Analysis of DNA-measurement of cell kinetics by the bromodeoxyuridine/anti-bromodeoxyuridine method

  • Analysis of cell proliferation using the bromodeoxyuridine/Hoechst-propidium iodide method

  • Chromosome analysis and sorting by flow cytometry

  • Intracellular ionised calcium, magnesium, membrane potential and pH

  • Flow cytometry in the study of apoptosis

  • Further applications to cell and molecular biology


Produktdetails

Erscheinungsdatum
18. Mai 2000
Sprache
englisch
Auflage
3. Auflage
Seitenanzahl
300
Autor/Autorin
Michael G. Ormerod
Herausgegeben von
Michael G. Ormerod, M. G. Ormerod
Verlag/Hersteller
Produktart
kartoniert
Gewicht
585 g
Größe (L/B/H)
246/189/16 mm
ISBN
9780199638246

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