HDAC/HAT Function Assessment and Inhibitor Development

Detection of Autophagy Induction After HDAC Inhibitor Treatment in Leukemic Cells. - Assessment of HDACi-Induced Protein Cleavage by Caspases. - Assessment of HDACi-Induced Cytotoxicity. - Analysis of HDACi-Induced Changes in Chromosomal Passenger Complex Localization. - Analysis of Histone Deacetylase-Dependent Effects on Cell Migration Using the Stripe Assay. - Using Histone Deacetylase Inhibitors to Analyze the Relevance of HDACs for Translation. - Effects of HDACi on Immunological Functions. - DNA Fiber Spreading Assay to Test HDACi Effects on DNA and Its Replication. - Assessing the Effect of Class I Histone Deacetylase Activity on DNA Double-Strand Break Repair by Homologous Recombination. - Establishment and Characterization of Long-Term Cultures Derived from Primary Acute Myeloid Leukemia Cells for HDAC Inhibitor Research. - Assessing the Histone Deacetylase Activity of SIRT6 in Primary Murine Hepatocytes Via Proximity Ligation Assay. - Purification and Analysis of Male Germ Cells from Adult Mouse Testis. - Generation of Tissue-Specific Mouse Models to Analyze HDAC Functions. - Engineering of Conditional Class I
Hdac
Knockout Mice and Generation of a Time-Spatial Knockout by a Dual Recombination System. - Xenografting of Cancer Cell Lines for In Vivo Screening of the Therapeutic Potential of HDAC Inhibitors. - Generation of Xenotransplants from Human Cancer Biopsies to Assess Anti-Cancer Activities of HDACi. - Phospho-Flow Analysis of Primary Mouse Cells after HDAC Inhibitor Treatment. - Evaluation of Innate Immune Gene Expression Following HDAC Inhibitor Treatment by High Throughput qPCR and PhosFlow Cytometry. - Assessing HDAC Function in the Regulation of Signal Transducer and Activator of Transcription 5 (STAT5) Activity Using Chromatin Immunoprecipitation (ChIP). - Modulation of STAT1-Driven Transcriptional Activity by Histone Deacetylases. - Proximity Ligation Assay to Quantify Foxp3 Acetylation in Regulatory T Cells. - Testing the Effects of SIAH Ubiquitin E3 Ligases on Lysine Acetyl Transferases. - Assessment of HDACi-Induced Acetylation of Non-Histone Proteins by Mass Spectrometry. - Assessing the Role of Paralog-Specific Sumoylation of HDAC1. - Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques. - How to Distinguish Between the Activity of HDAC1-3 and HDAC6 with Western Blot. - Evaluating the Effect of HDAC8 Inhibition in Malignant Peripheral Nerve Sheath Tumors. - Analyzing the Impact of Pan- and Class-Specific HDACi on Differentiation-Associated Factors. - Encapsulation of the HDACi Ex527 into Liposomes and Polymer-Based Particles. - Preparation of Histone Deacetylase Inhibitor Vorinostat Loaded Poly d, l-lactide-co-glycolide Polymeric Nanoparticles by Nanoprecipitation Method. - Generation and Assessment of Fusions Between HDACi and TKi.