This book describes the most important techniques used for studying cfDNA in the different samples; serum, plasma, urine. Chapters detail methods on liquid biopsy for cancer disease, methods in cancer, epigenetic modifications, fetal and pediatric diseases, physical activity, and urinary cell free DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge,
Cell-Free DNA as Diagnostic Markers: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Inhaltsverzeichnis
Cell Free DNA: Applications in Different Diseases. - Cell Free DNA: An Overview of Sample Types and Isolation Procedures. - Circulating Cell Free DNA and Cancer Therapy Monitoring: Methods and Potential. - Cell Free DNA in the Liquid Biopsy Context: Role and Differences between ctDNA and CTC Marker in Cancer Management. - Cell Free DNA Integrity: Applications. - Copy Number Variation Analysis on Cell Free Serum DNA. - Studying Copy Number Variations in Cell Free DNA: The Example of AR in Prostate Cancer. - dPCR Mutational Analyses in Cell-free DNA: A Comparison With Tissues. - From cfDNA to Sequencing: Workflows and Potentials. - Epigenetic Characterization of Cell Free DNA. - Quantitative Methylation-specific PCR: A Simple Method for Studying Epigenetic Modifications of Cell Free DNA. - Cell Free DNA in Pediatric Rhabdomyosarcoma: Potential and Challenges. - Bioinformatics Pipeline for Accurate Quantification of Fetal DNA Fraction in Maternal Plasma. - Circulating Cell-Free DNA in Physical Activities. - Urinary Cell Free DNA: Potential and Applications. - Urinary Cell Free DNA: Isolation, Quantification, Quality Assessment.
