HDAC/HAT Function Assessment and Inhibitor Development

Analyzing Lymphoma Development and Progression Using HDACi in Mouse Models. - Colony Formation Assay to Test the Impact of HDACi on Leukemic Cells. - Defined Human Leukemic CD34+ Liquid Cultures to Study HDAC/Transcriptional Repressor Complexes. - Development of a Cellular Model Mimicking Specific HDAC Inhibitors. - Evaluation of Anti-Tumor and On-Target Activity of HDAC Inhibitors with the Zebrafish Embryo Xenograft Model. - Human Platelet Lysate as Valid Cell Growth Additive to Assess Protein Acetylation. - Investigating Physiopathological Roles for Sirtuins in a Mouse Model. - Patient-Derived Organoids for In Vivo Validation of In Vitro Data. - Analysis of HDACi-Coupled Nanoparticles: Opportunities and Challenges. - Development of Pyrazine-Anilinobenzamides as Histone Deacetylase HDAC1-3 Selective Inhibitors and Biological Testing Against Pancreas Cancer Cell Lines. - Evaluation of Small Molecule HDAC Inhibitors through In Vitro and In Cellulo

Approaches. - Synthesis, Biochemical, and Cellular Evaluation of HDAC6 Targeting Proteolysis Targeting Chimeras. - HDACi Delivery Systems Based on Cellulose Valproate Nanoparticles. - Synthesis and Characterization of Reversible Covalent HDAC4 Inhibitors. - Analysis of Cell Cycle and DNA Compaction Dependent Subnuclear Distribution of Histone Marks. - Analyzing the Effects of HDAC Inhibitors on DNA Damage and Associated Cytotoxicity in Primary Hepatocytes. - Assessment of HDAC Inhibitor-Induced Endoplasmic Reticulum (ER) Stress. - Assessment of Mitochondrial Dysfunctions after Sirtuin Inhibition. - Assessing the Effect of Histone Deacetylase Inhibitors on DNA Double-Strand Break Repair by Non-Homologous End Joining. - Assessing SIRT7 Activity In Vivo and In Vitro in Response to DNA Damage. - Methods to Evaluate the Effects of HAT/KAT Inhibition on SIAH2-Driven Reactive Oxygen Species Generation in
Helicobacter pylori
-Infected Gastric Epithelial Cells. - Monitoring Changes in Intracellular Reactive Oxygen SpeciesLevels in Response to Histone Deacetylase Inhibitors. - Single-Cell Analysis of Histone Acetylation Dynamics at Replication Forks Using PLA and SIRF. - Testing the Effect of Histone Acetyltransferases on Local Chromatin Compaction. - A Genome-Wide CRISPR-Cas9 Loss-of-Function Screening to Identify Host Restriction Factors Modulating Oncolytic Virotherapy. - Cloning Strategy for HDAC1/HDAC2 Hybrid Protein Expression in Mammalian Cells. - Continuous Histone Deacylase Activity Assays. - CRISPR Activation/Interference Screen to Identify Genetic Networks in HDAC-Inhibitor Resistant Cells. - Determining Potency of Inhibitors Targeting Histone Deacetylase 6 by Quantification of Acetylated Tubulin in Cells. - Expression and Crystallization of HDAC6 Tandem Catalytic Domains. - Fluorescence Polarization-Based Competition Assays to Evaluate Histone Deacetylase 6 Inhibitors. - Immunoprecipitation of HDAC6 and Interacting Proteins.