The enormous advances in molecular biology and genetics coupled with the progress in instrumentation and surgical techniques have produced a voluminous and often bewildering quantity of data. The need for a second edition of Cardiovascular Physiology in the Genetically Engineered Mouse is underscored not only by these rapid advances, but by the increasing numbers of scientists who have focussed their research on genetically engineered mice. It is the primary objective of this second edition to interpret critically the literature and to provide a framework for the enormous amount of information in this burgeoning field. As in the first edition, the monograph serves as a practical guide for the investigator interested in the functional methods used to characterize the murine cardiovascular phenotype. However, this guidebook is a more comprehensive text than its predecessor; although the major objectives enumerated in the first edition have not substantially changed, they have been refined in keeping with the increased sophistication of the molecular biologist, geneticist, and physiologist in each other's discipline. Each chapter has been expanded and updated, richly enhanced with original tables and figures, and in many cases, extensively rewritten. Eight chapters written by internationally recognized experts have been added; this represents a 43 % increase from the first edition.
Inhaltsverzeichnis
One. - 1. The Hierarchical Approach to Analysis of Cardiovascular Function in Mice: An Overview. - 2. Genetic Alterations and Modeling of Cardiovascular Physiology. - 3. Conditional Transgenesis. - 4. Array Transcription Profiling: Molecular Phenotyping Of Rodent Cardiovascular Models. - 5. Murine Cardiovascular Development. - Two. - 6. Isolated Myocyte Mechanics and Calcium Transients. - 7. Single Cell Patch-Clamp Analysis of Mouse Cardiac Myocytes. - 8. The Isolated Isovolumic (Langendorff) Heart Preparation. - 9. The Isolated Work-Performing and Ejecting Mouse Heart Preparation, Comparison and Quantification of Cardiac Performance in Transgenic and Wild-type Mice. - 10. 31P NMR Spectroscopy of the Mouse Heart. - 11. Electrophysiological Characterization of the Mouse Heart Using Voltage-Sensitive Dyes. - 12. Echocardiographic Assessment of the Mouse Heart and Aorta. - 13. In Vivo Invasive Hemodynamic Studies in Mice. - 14. Pressure-Volume Relations. - 15. Assessment of Left Ventricular Diastolic Function in Mice. - 16. In Vivo Electrophysiologic Studies in Mice. - 17. Cardiovascular Physiology in Mice: Conscious Function Measurements and Effects of Anesthesia. - 18. Exercise and Oxygen Consumption in the Mouse. - 19. Clinical Laboratory Analysis of the Genetically Manipulated Mouse. - Three. - 20. Smooth Muscle Studies Using Gene-Altered Mouse Models: A Users Guide. - 21. Models of Cardiac Disease in the Mouse, Cardiac Hypertrophy and Heart Failure. - 22. Analysis of Murine Embryonic Cardiovascular Phenotype. - 23. Newer In Vivo Imaging Modalities.
