Bioconjugation Methods

This volume presents cutting-edge chemical and molecular strategies. Chapters detail bioorthogonal cycloadditions, electrophiles, self labeling systems, noncanonical amino acids, site selective bioconjugation across proteins, nucleic acids, cells, and viral assemblies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Bioconjugation Methods is a valuable resource for all researchers who are looking to learn more about this important and advancing field.

Site-specific chemical conjugation of recombinant proteins onto the AAV capsid. - Bioorthogonal Surface Modification of Measles and Vaccinia Viruses Using Metabolic Azide Reporters. - Synthesis of linkage-specific polyubiquitin probes using unnatural amino acids. - Synthesis of Ub-ACA, a Fluorogenic DUB Substrate via ACPL Method. - Native Chemical Ligation to Install Chemical Protein Modifications. - Efficient and Site-Specific Protein Bioconjugation Quantification in Mammalian Cells. - Intelligence disclosure on deploying SpyTag/SpyCatcher for Nanoparticle Assembly. - Rapid and Wash-free Live Cell Labeling with BenzoTag. - Antibody Conjugation to Cell Surface Receptors. - Cysteine Re-bridging Using Divinyl Pyrimidine Reagents for the Generation of Antibody-Drug Conjugates. - Sortase A Catalyzed Transpeptidation for Protein Bio-engineering. - Attachment of 2-Pyridyltetrazine Affinity Bioorthogonal Chemistry (ABC) Tags to Proteins for Site-selective Bioorthogonal Chemistry. - N-acyl-N-alkyl sulfonamide mediated bioconjugation of His-tag fused proteins. - Chemoselective Histidine Bioconjugation Using Thiophosphorodichloridate Reagents. - Reactive DNA Probes for Cross-Linking with Specific Amino Acids of Protein. - Protein Modification via Bioorthogonal HS-BCN Ligation.