Arbuscular Mycorrhizal Fungi

This detailed new edition presents current techniques for researching arbuscular mycorrhizal (AM) fungi. With an updated and expanded collection of protocols, this book explores molecular quantification, high resolution imaging, metabolite profiling, transcriptomic and proteomic analyses, functional genomics, and advanced cultivation systems. Methods for asymbiotic growth and the study of functional traits of AM fungi and techniques to describe microbial communities, including viruses, associated with AM fungal hyphae are also included. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Arbuscular Mycorrhizal Fungi: Methods and Protocols, Second Edition serves as a vital resource for researchers working in different areas of AM fungal biology.

Methods for Single-Spore Cultures of Arbuscular Mycorrhizal Fungi Using a Superabsorbent Polymer-Based Autotrophic System. - Asymbiotic Growth of Arbuscular Mycorrhizal Fungi. - A Whole-Plant Culture Method to Study Structural and Functional Traits of Extraradical Mycelium. - In Vitro Culture Systems to Study the Functions of Common Mycorrhizal Network in Arbuscular Mycorrhizal Symbioses. - Histochemical Staining of Arbuscular Mycorrhizal Roots for Quantification of Fungal Colonization, High-Resolution Imaging, and Localization of Symbiotic Gene Expression. - Intracellular Inorganic Orthophosphate Distribution in Arbuscular Mycorrhizal Root. - Quantification of Arbuscular Mycorrhizal Symbiosis by Molecular and Gene Expression Analysis (qRT-PCR). - Collection and Purification of Secondary Metabolites from Root Exudates for LC-MS/MS Analyses. - Standardizing Methods for Shoot Metabolomics in Mycorrhizal Plants. - Purification and Analysis of Signaling Molecules in Plant-AMF Communication. - Recovery of Extra-Radical Fungal Peptides Amenable for Shotgun Protein Profiling in Arbuscular Mycorrhizae. - Collection of Arbuscular Mycorrhizal Hyphal Exudates Under In Vitro Culture Conditions. - Characterization of Bacteria Living on Arbuscular Mycorrhizal Fungal Hyphae Under Field Conditions. - Investigating RNA Viruses Infecting Arbuscular Mycorrhizal Fungi. - From Sampling to Identification of AMF Through Next Generation Sequencing. - Metagenomics Approach for Identification of AMF Sequences in Historical Herbarium Specimens. - Methods for Arbuscular Mycorrhizal Fungal Genome Characterization. - Transcriptome Analysis of Arbuscular Mycorrhizal Fungi Using Short-Read Sequencing. - Co-Extraction of Small RNAs and Total RNA from Arbuscular Mycorrhizal Roots Using Laser Microdissection. - Application of Virus-Induced Gene Silencing to Arbuscular Mycorrhizal Fungi.