Serving as a useful resource for undergraduate microbiology laboratory courses, this book is intended for either a majors or non-majors lab course.
Inhaltsverzeichnis
Laboratory Protocol
Safety
1 Microscopy
1 Brightfield Microscopy
2 Darkfield Microscopy
3 Phase-Contrast Microscopy
4 Fluorescence Microscopy
5 Microscopic Measurements
2 Survey of Microorganisms
6 Protozoans, Algae, and Cyanobacteria
7 Ubiquity of Bacteria
8 The Fungi: Yeasts and Molds
3 Manipulation of Microorganisms
9 Aseptic Technique
10 Pure Culture Techniques
4 Staining and Observation of Microorganisms
11 Smear Preparation
12 Simple Staining
13 Negative Staining
14 Capsular Staining
15 Gram Staining
16 Spore Staining: Two Methods
17 Acid-Fast Staining: Ziehl-Neelsen Method
18 Motility Determination
5 Culture Methods
19 Culture Media Preparation
20 Preparation of Stock Cultures
21 Cultivation of Anaerobes
22 Enumeration of Bacteria: The Standard Plate Count
23 Slime Mold Culture
24 Slide Culture: Molds
6 Bacterial Viruses
25 Determination of a Bacteriophage Titer
26 Burst Size Determination: A One-Step Growth Curve
27 Isolation of Phage from Flies
28 Phage Typing
7 Environmental Influences and Control of Microbial Growth
29 Temperature: Effects on Growth
30 Temperature: Lethal Effects
31 pH and Microbial Growth
32 Water Activity and Osmotic Pressure
33 Ultraviolet Light: Lethal Effects
34 Oligodynamic Action
35 Evaluation of Alcohol: Its Effectiveness as an Antiseptic
36 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method
37 Evaluation of Antiseptics: The Filter Paper Disk Method
38 Effectiveness of Hand Scrubbing
8 Identification of Unknown Bacteria
39 Morphological Study of Unknown Bacterium
40 Cultural Characteristics
41 Physiological Characteristics: Oxidation and Fermentation Tests
42 Physiological Characteristics: Hydrolytic Reactions
43 Physiological Characteristics: Biochemical Tests
44 Use of Bergey's Manual
9 Miniaturized Multitest Systems
45 Enterobacteriaceae Identification: The API 20E System
46 Enterobacteriaceae Identification: The Enterotube II System
47 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System
48 Staphylococcus Identification: The API Staph-Ident System
10 Diversity and Environmental Microbiology
49 Isolation of an Antibiotic Producer: The Actinomyces
50 The Nitrogen Cycle: Amonification
51 Symbiotic Nitrogen Fixation: Rhizobium
52 Free-living Nitrogen Fixation: Azotobacter
53 Denitrifcation: Paracoccus denitrificans
54 The Winogradsky Column
55 Purple Nonsulphur Photosynthetic Bacteria
56 Sulfate Reducing Bacteria: Desulfovibrio
57 Bacterial Commensalism
58 Bacterial Synergism
59 Microbial Antagonism
11 Applied Microbiology
60 Bacterial Counts of Foods
61 Bacterial Examination of Water: Qualitative Tests
62 The Membrane Filter Method
63 Reductase Test
64 Microbial Spoilage of Canned Food
65 Microbiology of Alcohol Fermentation
12 Bacterial Genetics and Biotechnology
66 Mutant Isolation by Replica Plating
67 Bacterial Transformation
68 Polymerase Chain Reaction for Amplifying DNA
69 Plasmid Isolation
13 Medical Microbiology and Immunology
70 The Staphylococci: Isolation and Identification
71 The Streptococci: Isolation and Identification
72 Gram Negative Intestinal Pathogens
73 Slide Agglutination Test: Serological Typing
74 Slide Agglutination (Latex) Test: For S. aureus Identification
75 Tube Agglutination Test: The Heterophile Antibody Test
76 White Blood Cell Study: The Differential WBC Count
77 Blood Grouping
78 A Synthetic Epidemic
Appendixes
A Tables
B Indicators, Stains, Reagents
C Media
D Identification Charts
E The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics
