Several approaches, such as microarray hybridization, have become extremely popular tools for specialists in biochemistry and biomedicine, while the potential of many other advantageous techniques seems to be underestimated. Written by an international team of authors, this book details the current state-of-art in hybridization techniques. Coverage includes experimental protocols along with comprehensive and detailed method descriptions.
Inhaltsverzeichnis
Nucleic Acids Hybridization: Potentials and Limitations. - Selective Suppression of Polymerase Chain Reaction and Its Most Popular Applications. - Suppression Subtractive Hybridization. - Stem-Loop Oligonucleotides as Hybridization Probes and Their Practical Use in Molecular Biology and Biomedicine. - Normalization of cDNA Libraries. - Primer Extension Enrichment Reaction (PEER) and Other Methods for Difference Screening. - Subtractive Hybridization with Covalently Modified Oligonucleotides. - Coincidence Cloning: Robust Technique for Isolation of Common Sequences. - DNA Hybridization in Solution for Mutation Detection. - Current Attempts to Improve the Specificity of Nucleic Acids Hybridization. - Concepts on Microarray Design for Genome and Transcriptome Analyses.
