The 9th Symposium on Analytical Ultracentrifugation (AUC) which was held at the Max Delbrück Center for Molecular Medicine in Berlin-Buch, March, 1995, is the basis of this volume. It presents an actual survey of recent developments and results of ultracentrifugation methods in the frame of the following topics: Reversible Association Reactions. - Analysis of Supramolecular Structures Including Modeling. - Size Distribution Analysis and Gel Formation. - Hardware and Software Developments.
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Self-association of the molecular chaperone HSC70 as assessed by analytical ultracentrifugation. - Investigation of the molecular chaperone DnaJ by analytical ultracentrifugation. - Investigations of the oligometric state of the 42 kDa repressor isoform from the streptomcyes temperate bacteriophage ? C31. - Characterisation of the solution structure of halophilic proteins. Analytical centrifugation among complementary techniques (light, neutron and X-ray scattering, density measurements). - ? -helical coiled coils: simple models for self-associating peptide and protein systemss. - Improved approach for characterizing the coalescence stability of legumin stabilized O/W emulsions by analytical ultracentrifugation. - Preliminary ultracentrifuge studies of the polyelectrolyte behaviour of Welan gum. - Netropsin-induced changes of DNA supercoiling; sedimentation studies. - Sedimentation studies of specific association of oligonucleosomes from sea urchin sperm chromatin. - Complex formation and crystallization of adrenodoxin-reeuctase with adrenodoxin. - Studying heterologous associations between membrane proteins by analytical ultracentrifugation: Experience with erythrocyte band 3. - Chemical cross-linking and analytical ultracentrifugation study of the histone-like protein HBsu: Quaternary structure and DNA binding. - Hydrodynamic modelling of the solution conformation of 10 S myosin. - Supramolecular structure of the small heat shock protein Hsp25. - Physical-chemical characterization of the different individual cortical alfa-crystallin fractions from bovine lenses. - Sedimentation parameter of linear polymers. - Velocity sedimentation of water-soluble methyl cellulose. - Simultaneous determination of particle and density distributions of dispersions by analytical ultracentrifugation. -Molar mass distribution of polymers from sedimentation velocity in an analytical ultracentrifuge. - Molar mass distribution from sedimentation fractiometry (SF)-competition with size exclusion chromatography (SEC, GPC). - Reaction kinetic and molar mass distribution of the polycation poly[(dimethyleneimino)ethylene-(dimethyleneimino)-methylene-1, 4-phenylenemethylenedichloride]. - Direct digital data capture for sedimentation velocity experifments using UV/VIS optics. - Studies of microgel formation in aqueous and organic solvents by light scattering and analytical ultracentrifugation. - Formation of reversible concentration gradients during the centrifugation of gels. - Measurement of the extinction coefficient of prostate specific antigen using interference and absorbance optics in the Optima XL-A analytical ultracentrifuge. - A study on Schlieren patterns derived with the Beckman Optima XL-A UV-absorption optics. - A comparative Schlieren study of the sedimentation behaviour of three polysaccharides using the Beckman Optima XL-A and model E analytical ultracentrifuges. - Alternative light sources for the Schlieren optical system of analytical ultracentrifuges. - The determination of liquid/liquid interfacial mass transfer A new application for the analytical ultracentrifuge.
