Autoantibodies

This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Autoantibodies: Methods and Protocols aims to be helpful for all persons working with research and development of autoimmune laboratory diagnostics and for clinicians using autoantibody tests in daily work with patients.

Autoantibodies as Diagnostic Tools. - Non-specific Binding in Immunoassays for Autoantibodies. - Detection of Autoantibodies by Indirect Immunofluorescence Cytochemistry on HEp-2 Cells. - Detection of Anti-Neutrophil Cytoplasmic Antibodies by Indirect Immunofluorescence. - Determination of Subset-Restricted Anti-Neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry. - Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry. - Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry. - Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay. - Determination of Autoantibodies to Salivary Gland Antigens. - Measurement of autoantibodies to the gastric H+, K+-ATPase (ATP4A/B) using a Luciferase Immuno Precipitation System (LIPS). - Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay. - Determination of Autoantibodies to Neutrophil Granule Constituents by Solid Phase Immunoassay. - Determination of Autoantibodies to C3 Nephritic Factors by ELISA. - Determination of Anti-C1q Autoantibodies by Western Blot. - Anti-Factor H Autoantibodies Assay by ELISA. - Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay. - Determination of CRP Autoantibodies by SPR Immunoassay. - Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis. - Detection of SSA and SSB Autoantibodies by Enzyme-Linked Immunosorbent Assay. - Assessment of Peptidylarginine Deiminase Activity by ELISA using Human Fibrinogen as Substrate. - The Use of a Citrullinated Peptide Panel for Detection of Anti-Citrullinated Protein Antibodies by Enzyme-Linked Immunosorbent Assay. - Determination of Autoantibodies by Line Blotting. - Determination of Rheumatoid Factors by ELISA. - Detection of autoantibodies to complement components by surface plasmon resonance-based technology.